gel retardation assay method?

Doug-young Ryu dyryu at unity.ncsu.edu
Wed Feb 22 21:13:08 EST 1995


i am trying to perform gel retardation assay (mobility shift assay) with 
liver tisse nuclear extracts.  It  has so far given non specific bands 
only.  

If somebody has good protocol for this technique, please give me some 
good advice.  (from nuclear extraction from liver to running PAGE)

thanks in advance.

dyryu at unity.ncsu.edu




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