HELP NEEDED FOR pfu DNA POLYMERASE

Brian Carey bcarey at zookeeper.zoo.uga.edu
Wed Feb 22 19:55:09 EST 1995


In article <3i6gqc$ohe at hydra.acs.ttu.edu>, Z3C20 at ttacs3.ttu.edu (Yi,
Xiaoming) wrote:

> I am a using pfu DNA pol(Stratagene) to amplify a 400bp sequence with a 18mer
> and a 500bp PCR fragment as the other primerr. I could not get enough product.
> Can anybody in this group tell me what I should do? I am using the 10X buffer
> with the enzyme and standard conditions.
> Any help will be appreciated.

Question: Is your DNA template used in the PCR reaction linear?
I get poor yield with circularized plasmid. Linear plasmid gave better results.

Rita
Athens, UGA



More information about the Methods mailing list