AMOUNT OF PRIMER IN DNA FINGERPRINTING

GORDON BETTS betts at ORION.ETSU.EDU
Wed Feb 22 14:51:24 EST 1995


I am about to order some primer for an undergraduate fingerprinting exercise
I am trying to duplicate and need to know how much primer to use.  I will
use the D1S80 primer.  The procedure by Budowle et al. (Am. J. Hum. Genet.
48:137) calls for 1 uM.  Isn't this a lot?  When I look at order forms, they
often have a checkbox for 50 or 100 nM.  Lot of difference!  
Thanks!
Gordon Betts
Biology Dept.
East Texas State University
Commerce, TX 75429-3011
903/886-5369
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