single-stranded DNA in S1 mapping

Toshiharu Ishizuka tishizuk at AQUARIUS.BEKKOAME.OR.JP
Wed Feb 22 07:42:20 EST 1995

Dear netters

        I'm afraid this is a primitive question. Does anybody know why we
have to add single-stranded calf-thymus DNA to the S1 nuclease digestion
buffer in S1 nuclease mapping of mRNA. Without the DNA, what will happen ?
I will greatly appreciate it if somebody teach me. Sorry again for this
stupid question ?

Sincerely yours,

Dr. Toshiharu Ishizuka
Department of Biochemistry, Chiba University, School of Medicine
1-8-1, Inohana, Chuo-ku, Chiba, 260 JAPAN
FAX +81-43-226-2041
QFH03407 at
tishizuk at

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