EtBr in agarose gels vs. EtBr staining later
rmoldwin at midway.uchicago.edu
rmoldwin at midway.uchicago.edu
Thu Feb 23 09:59:54 EST 1995
In article <Pine.A32.3.91.950222182822.74692A-100000 at rs5.tcs.tulane.edu>, Brick
Ayola <abrick at mailhost.tcs.tulane.edu> writes:
>
>Regarding EtBr staining of nucleic acids:
>Can one simply add EtBr to the loading dye (as with some RNA gel
>protocols)? Or will this significantly disturb the migration of DNA
>super-coils and such?
>
>Brick Ayola, Ph.D.
>Tulane School of Medicine
If I recall correctly, when I tried it, the EtBr migrated in the opposite
direction as the DNA. Also, when you add EtBr to the gel, you can see that the
bottom of the gel is always darker than the top. I think this is because the
EtBr migrates upward. The implication is that low bp bands at the bottom of
the gel may not show up under UV light. Am I right about this
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