Primer design and Pfu/Pwo polymerases fro PCR

Alistair Forrest forrest at
Thu Feb 23 20:53:20 EST 1995

G'day all,
	I want to use Pwo (similar activities to Pfu) to amplify some
cDNA. For a number of PCrs i want to add extra sequence 5' to the region
of hybridization. HAs anyone had trouble with the exonuclease activity chomping
up the 5' end of the primer??

Thanks for any feedback,
	Alistair Forrest (PhD student)

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