Elution of immunoaffinity isolated protein with peptide

Dayanidhi Raman maraman at ksu.ksu.edu
Thu Feb 23 18:31:52 EST 1995

I am trying to elute a protein that has been immunoaffinity isolated with the peptide against which the antibody has been made. I tried a range of peptide levels (0.3 mg/ml - 5mg/ml) at two time points (4 h and overnight) and did not elute even very little protein. I need help in this regard.

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