Details on CNBr Cleavage

CTHOMPSON at ARSERRC.GOV CTHOMPSON at ARSERRC.GOV
Thu Feb 23 16:17:44 EST 1995


Hello,
I was wondering if anyone could direct me to the correct use of a 
Cyanogen Bromide Activated Matix on Sepharose 4B (by Sigma) which is
lactose and Dextran.  I would like to cleave collagen at its methionine
sites but I don't know if I can hydrate the Sepheraose with solutions
with a pH around 2.0.  Will the cyanogen bromide come off into solution at
that pH?

Craig



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