Bacteria cell lysis

schupp at nauvax.ucc.nau.edu schupp at nauvax.ucc.nau.edu
Fri Feb 24 10:43:47 EST 1995


Tari,
        You might try Polymyxin B sulfate (Sigma).  It has been used with
gram- cells to purify proteins at 2mg/ml, 4C.  The cells will lyse in about
30 min. at room temp.  I use it to permeabilyze cells, however I have no
experience with protein purification.

Jim Schupp



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