PBR322 Sequence?

Tracy Aquilla aquilla at salus.med.uvm.edu
Fri Feb 24 08:08:02 EST 1995


In Article <1995Feb22.190548.30457 at ucl.ac.uk>, regokag at ucl.ac.uk (Dr Keith
Anthony Grimaldi) wrote:
>ovanham at julian.uwo.ca (o.   van-ham) writes:
>
>>In article <Rosenwasserlab-150295152351 at 198.243.65.232>,
>>Calvin and Hobbbes <Rosenwasserlab at njc.org> wrote:
>>>Anyone have a sequence/restriction digest map for PBR322? I have tried a
>>>blast search with NIH Library and have received only genes cloned into PBR
>>>322. Or if it is published in a catalog that would be great too. I realize
>>>that this vector is pre-historic, but I have a gene that I want to sequence
>>>in it.
>>>
>>>Thanks for your help,
>>>
>>>Jon Stacks
>
>
>
>>Check out the New England Biolabs book.  It has the pBR322 restriction 
>>digest map, but no sequence (sorry, can't help with that).
>
>>Oded Van-Ham
>
>
>If I remember correctly the original (1982?) edition of Maniatis has the
>entire sequence of PBR322. If you cannot find it mail me and I will send you a
>copy (k.grimaldi at ucl.ac.uk). If by any chance anybody in my lab happens to
>have the sequence I can e-mail it. Let me know if you need it.
>
>Keith Grimaldi
>
>
>--
>        Keith Grimaldi
>        Dept. Oncology
>        University College London       TEL: (0)171-636-8333  ext:3419
>        91, Ridinghouse Street

Maybe this will help: LOCUS SYNPBR322V; ACCESSION # L08654; SOURCE Synthetic
construct DNA; J.G. Sutcliffe, Cold Spring Harbor Symp. Quant. Biol. (1978)
43:77-90. Good luck.
    tracy



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