Re-using hybridisation solutions containing double-stranded probes?

DREWES at MPASMB.DESY.DE DREWES at MPASMB.DESY.DE
Fri Feb 24 19:37:59 EST 1995


Has anybody ever tried re-using plaque hybridisation solutions containing
32P-labeled double stranded probes? It would mean a significant reduce in
radioactive waste and expensive stuff like salmon sperm DNA and deionized
formamide. I am thinking about simply storing the used solution in the freezer
and then denature the DNA and the probe again by boiling for ten minutes before
the next use.
Thanks for any suggestions...


*****************************************************************
*  Gerard Drewes                          DREWES at MPASMB.DESY.DE *
*  Max-Planck-Society                     Tel.(+49)40-89982820  *
*  Structural Molecular Biology Unit      Fax (+49)40-891314    *
*  DESY, Notkestr. 85                                           *
*  22603 Hamburg, Germany                                       *
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