Details on CNBr Cleavage

[XYZZYX at DELPHI.COM]

CTHOMPSON at ARSERRC.GOV writes:

>Hello,
>I was wondering if anyone could direct me to the correct use of a 
>Cyanogen Bromide Activated Matix on Sepharose 4B (by Sigma) which is
>lactose and Dextran.  I would like to cleave collagen at its methionine
>sites but I don't know if I can hydrate the Sepheraose with solutions
>with a pH around 2.0.  Will the cyanogen bromide come off into solution at
>that pH?

>Craig

This matrix was designed for immobilization of proteins.  You will not 
get much back if you load protein onto this gel.  Why not just dump CnBr 
into a tube of reaction buffer and protein?