Details on CNBr Cleavage

XYZZYX at DELPHI.COM XYZZYX at news.delphi.com
Sat Feb 25 01:51:58 EST 1995


CTHOMPSON at ARSERRC.GOV writes:

>Hello,
>I was wondering if anyone could direct me to the correct use of a 
>Cyanogen Bromide Activated Matix on Sepharose 4B (by Sigma) which is
>lactose and Dextran.  I would like to cleave collagen at its methionine
>sites but I don't know if I can hydrate the Sepheraose with solutions
>with a pH around 2.0.  Will the cyanogen bromide come off into solution at
>that pH?

>Craig

This matrix was designed for immobilization of proteins.  You will not 
get much back if you load protein onto this gel.  Why not just dump CnBr 
into a tube of reaction buffer and protein?





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