Elution of immunoaffinity isolated protein with peptide

Anne Spang spang at vms.biochem.mpg.de
Sun Feb 26 07:03:51 EST 1995


Could you pleas be a little bit more specific? Is your protein bound to
antibodies? Then you will not be able to elute them with a peptide. The
antibody-antigene interaction is to strong. You could get your protein out
of the column with 0.2 M glycine pH 2.5-3.0 or 0.1 M ethanolamine pH
9.0.For the elution at low pH, you must adjust the pH immediately. If you
are lucky, you might get ´native´ protein.

Hope this helps

Anne



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