T3/T7 RNA-Polymerases

Uwe Harmening harmening at cellbiology.uni-frankfurt.d400.de
Thu Feb 23 13:44:43 EST 1995


Hi Netters,

how is the yield of an in vitro transcription reaction with t3 or t7 rna-
polymerases related to the length of the template? Is it as easy as: the
shorter the template, the better the outcome? My experience is that for
a shorter template i get more transcript (weight/volume). Are there any 
publications on this topic? I tried to find some in medline, but there were
to many as to browse through all off the abstracts :-()
Any comment welcome.

Thanx
Jui



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