The truth about Okazaki fragments?

Vic Yuschuk vy001d at uhura.cc.rochester.edu
Mon Feb 27 20:52:16 EST 1995


In <3is5fb$1qj at mserv1.dl.ac.uk> (Dave Johnston) <daj at mailserver.nhm.ac.uk> writes:

>Hi all,
>A quick question about DNA replication. Do Okazaki fragments occur on one 
>or both new strands during DNA replication?. Theoretically, they would only 
>be needed on the new strand whose 3' end doesn't follow the replication 
>fork, but some textbooks say they occur on both new strands anyway, 
>others claim they only occur on the one new strand.

>IE: IS IT:

>   __________________________
> 5'                          \
>   <--- <--- <--- <--- <---    \_____________________3'
> 3'                         5'                        
>                                _____________________
> 5' ----------------------->3' /                    5' 
>   __________________________/
> 3'

>OR
>   __________________________
> 5'                          \
>   <--- <--- <--- <--- <---    \_____________________3'
> 3'                         5'                        
>                                _____________________
> 5' ---> ---> ---> ---> --->3' /                    5' 
>   __________________________/
> 3'

>Thanks in advance
>DAJ

>David A. Johnston
>Research Fellow,
>Dept of Zoology, The Natural History Museum, Cromwell Road,
>South Kensington, London SW7 5DB. England
>(tel 071 9389297, fax 071 9388754, email daj at nhm.ac.uk)


The first diagram is right in general.  The text may have been referring to
Okazaki's original hypothesis in his '69 paper.  He thought the two strand
mechanism might be likely.  (I know you're smiling if you see this Dr.
Eickbush)

Vic Yuschuk



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