Specific RNAase aviable? - Reply

Don Jackson djackson at welchlink.welch.jhu.edu
Mon Feb 27 18:27:55 EST 1995

In article <BA99492F01B938D9 at office.mmc.org>, varyc.mmcri at OFFICE.MMC.ORG
(Varyc) wrote:

> Hi Min and all,
> Min writes:
> ..."but does anybody know if there is such specific RNAase which
> cuts RNA with a specific RNA sequence recognization just like a
> DNA restructaion endonuclease cutting DNA fragment? Is this kind of
> RNAase identifed or cloned?"
> The only generalizable approach that I know to sequence specific RNAsing is to direct RNAseH to a
> specific RNA sequence with a DNA oligo. See me, JBC 1984, v259, p3299-3307 and Hellen Donis-Keller,
> NAR 7, 179-192.... (rest deleted)

How about a ribozyme?

Don Jackson
Dept. of Molecular Biology and Genetics
Johns Hopkins University School of Medicine
djackson at welchlink.welch.jhu.edu

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