Highly competent cells

Steven Goldberg goldberg at bms.com
Mon Feb 27 15:56:40 EST 1995

mbxrh at unicorn.ccc.nottingham.ac.uk (mbxrh) wrote:
> I have a method for producing electrocompetent cells with a high
> efficiency. Etc...

I don't know what method you're using but I have found resuspending
the electrocompetent cells in ice-cold 10% glycerol and quick freezing aliquots
on dry ice works well.  No significant decrease in transformation
efficiency has been seen over several months of storage at -70oC.

Steve Goldberg

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