EtBr in gel vs. staining later

hodges bradley l segdoh at uxa.cso.uiuc.edu
Tue Feb 28 11:54:03 EST 1995


In my opinion DNA bands are more bright and distinct when EtBr is in the 
gel during electrophoresis.  Staining the gel after electrophoresis 
allows small DNAs to diffuse, creating less distinct bands.


On 22 Feb 1995, GORDON BETTS wrote:

> It seems to me there are two options for visualizing DNA in agarose gels:
> run a gel with EtBr in the gel or staining the gel after the run.  What are
> the relative merits of the two methods?
> Thanks,
> Gordon Betts
> Biology Dept.
> East Texas State University
> Commerce, TX 75429-3011
> 903/886-5369
> ============================================================================
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> Life is like a bowl of stew, you need to stir things up once in a while so 
> the scum doesn't rise to the top.
>       Author unknown
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