krasel at alf.biochem.mpg.de
Tue Jan 3 13:40:26 EST 1995
Shahram Mori (smori at nmsu.edu) wrote:
> Nadesalingam N Palaniyar (npalaniy at uoguelph.ca) wrote:
> : I am looking for an enzyme(s) or chemical(s) that could cleave the
> : phospho-tyrosine bond. This info I am looking for is needed for removing
> : a protein covalently bound to the DNA. This protein presumably binds the
> : DNA at the 3'-phosphate through the -OH group in the tyrosine of the
> : protein.
> This is just an idea, have no idea if it will work; Would alkaline Phosphatase
> work for this? I know it is used to remove the 5'phosphates from DNA or RNA.
> I wonder if it would work in this case!
I think you can either use Alkaline Phosphatase or Acid Phosphatase (which
is also available commercially) to dephosphorylate proteins on Serine or
Threonine residues. I am not sure about proteins phosphorylated on Tyrosine.
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de fax: +49 89 8578 3795 */
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