Self religation of double-cut plasmid vectors

mahmoud dbell at julian.uwo.ca
Tue Jan 3 21:47:14 EST 1995


Hi 

I was wondering in your hands, if you purify your double digested plasmid 
vector (any plasmid vector), and run a self religation experiment, what sort 
of transformation rate do you see? (how many colonies?) Of course I need to 
know how this rate is compared to the transformation rate of your non-cut 
plasmid control?  
In theory it should be zero, but...?


Thanks in advance

mahmoud  



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