Beta-Actin good RT-PCR Control?
mkennedy at chmeds.ac.nz
Tue Jan 3 20:18:54 EST 1995
In article <3e7ts0$t4o at dns1.NMSU.Edu>, smori at nmsu.edu (Shahram Mori) writes:
> How about using succinate dehydrogenase as the control. This one should always
> be there.
I, like others (who have also posted on the net) am always keen to find better
RT-PCR controls. I'm not familiar with succinate dehydrogenase - can you
please post more details, like PCR primers, whether applicable to both
mouse/human, whether pseudogenes are a problem etc. I'd be very grateful...
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-0750
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