Yeast Dropout Media

Stephanie L. Fox slf8 at po.CWRU.Edu
Tue Jan 3 18:35:12 EST 1995


I need to make minimal yeast dropout media.  I have a recipe
which calls for yeast nitrogen base w/o amino acids (Difco).
I have on hand yeast nitrogen base w/o amino acids and ammonium
sulfate.  Will I need to add ammonium sulfate to the media, and
if so, how much?

This is my first experience working with yeast.  I'd appreciate
any help you could give me.  Thanks.



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