Chloroform colony lysis

Peter Andrew Emanuel pemanuel at umabnet.ab.umd.edu
Wed Jan 4 09:01:27 EST 1995


Dear Ferenc,
  I have received several responses concerning the chloroform colony 
lysis procedure.  Some included a step in which the filter was treated 
with lysozyme, DNase and RNase.  Have you ever heard of such a thing?  
One simple procedure suggested that I lay pipets in a container and pour 
Chloroform on the bottom.  The filter was then laid on top of the pipets 
so that it did not contact the solution.  Following treatment with 
chloroform, what follows?
   Peter



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