Mike Morris mike at
Wed Jan 4 12:56:43 EST 1995

  We are starting RT-PCRs on illegitimate transcripts in very small
tissue/cell samples. HELP! 
  Q1/ 	Does anybody have experience of reliable RNA production from the 
	equivalent of 10^5-10^6 cells?
  Q2/   Any feelings about the relative advantages (in terms of reliability
	and sensitivity) of reverse transcriptases and thermostable 
	polymerases for reverse transcription?
  thanks in advance,

Division of Medical Genetics       tel (Switzerland) (22) 702.56.94
CMU, University of Geneva          fax (Switzerland) (22) 702.57.06
Geneva, Switzerland                email mike at                  

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