SENSITIVE RT-PCR

Mike Morris mike at medsun.unige.ch
Wed Jan 4 12:56:43 EST 1995


  We are starting RT-PCRs on illegitimate transcripts in very small
tissue/cell samples. HELP! 
  Q1/ 	Does anybody have experience of reliable RNA production from the 
	equivalent of 10^5-10^6 cells?
  Q2/   Any feelings about the relative advantages (in terms of reliability
	and sensitivity) of reverse transcriptases and thermostable 
	polymerases for reverse transcription?
  thanks in advance,
	Mike

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Division of Medical Genetics       tel (Switzerland) (22) 702.56.94
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