FTIR of DNA
akopian at cheshire.oxy.edu.
Thu Jan 5 17:22:47 EST 1995
If anybody out there has experience with FTIR of DNA, and or
making DNA samples for spectroscopic investigation, could you please send
a protocol of DNA sample preparation. Specifically, I have read from
published material, that most FTIR investigators of DNA make a 2% to 7%
w/w solution using .1M NaCl solution. I've tried this, and have only
managed to get a gel instead of a homogeneous liquidy solution. We cannot
use this gel, since our FTIR is equiped with an ATR Circle Cell. Use of a
gel creates unknown conditions in the chamber, especially since we do not
know if bubbles are present, and whether or not adsorbtion onto the
crystal takes place. Thanks in advance.
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