HeLa extracts for SV40 DNA replication

wick at cris.com wick at cris.com
Thu Jan 5 14:41:07 EST 1995

Does anyone have a good procedure for preparation and storage of HeLa cell extracts for SV40 DNA replication. We have tried 
the Li/Kelly and Wobbe/Hurwitz procedures and have low initial activity that rapidly is lost upon storage at -70C.

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