When Low Melt bands are contaminated???

Peter pemanuel at umabnet.ab.umd.edu
Thu Jan 5 14:19:45 EST 1995


In article <3ehbn0$q0t at mark.ucdavis.edu>, szcooley at dale.ucdavis.edu
(Michael Cooley) wrote:

> Peter (pemanuel at umabnet.ab.umd.edu) wrote:
> : I recently ran a 1% low melt gel isolation of a 700 bp PCR fragment. When
> : I cut out the band and isolated it with GENECLEAN I found that a
> : contaminating band of 1400 bp had gotten in there somehow.
> 
> 
> Why are you using Low Melt agarose if you are using GeneClean for the 
> isolation? Low Melt agarose is problematic. It is very true that loading 
> too much or running it too hot will cause a loss of resolution. 
> 
>
Dear Mike,
  I mistyped myself.  It was not low melt-it was simply a 1% TAE gel.  The
toothpick assay won't apply here since I want to pool all the PCR
reactions to produce an antibody phage display library.  Sorry about the
mis-type.
  Peter



More information about the Methods mailing list