Does CTAB effect 260nm readings?

Michael Cooley szcooley at dale.ucdavis.edu
Thu Jan 5 13:26:48 EST 1995


Sebastian W. Bunka (seb at i102pc1.vu-wien.ac.at) wrote:
: Paul A. Vescio (vescip at rpi.edu) wrote:
: [ stuff deleted ]
: :     As an aside: a 260nm reading is not as accurate as you may think in
: : estimating DNA concentration.   If you have access to one' a fluorometer
: : would give you a much better indication of your DNA concentration 
: Shure about this ? There was a thread in this newsgroup about this 1 year
: ago that summarizes that the fluorometer are too faulty and inaccurate.

: Has the technique been improved ??

: Just a thought ;-)
: Sebastian
: --

: email:                   [ Sebastian.Bunka at vu-wien.ac.at ]
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: Location: earth, europe, austria, vienna  Inst. of Bacteriology  Vet.Univ.


I hesitate to mention it but some people do not ever read the manuals 
that come with a piece of equipment. Fluorimeters are not trivial to use. 
If you follow the procedures carefully they work fine and very reliably.


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