HA epitope/immunogold

Anne Spang spang at vms.biochem.mpg.de
Fri Jan 6 10:07:54 EST 1995


Hi Jon!
I did Immuno-EM with 12CA5 on yeast cells and isolated protein complexes
embedded in LR-White. I fixed the cells for 30 min with 0.075%
glutaraldehyde and 4% formaldehyde in 40 mM phosphate buffer pH 6.4
containing 0.6M sorbitol. It worked out fine! We used teh antibody in a
1:250 dilution!

Hope this helps!
Anne



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