When Low Melt bands are contaminated???

Colin Rasmussen ras-1 at bones.biochem.ualberta.ca
Sun Jan 8 12:56:27 EST 1995


In article <1995Jan8.081711.10938 at lugb.latrobe.edu.au>,
BOTJLH at LURE.LATROBE.EDU.AU (HEAZLEWOOD,Joshua) wrote:

> In <pemanuel-0501950943340001 at beaker.ab.umd.edu>
pemanuel at umabnet.ab.umd.edu writes:
> 

> 
> Try heating the sample to 65C for a few minutes before loading. I have heard
> that this can be used to stop piggy-backing of the 4.3 kb band to the
6.5 kb in
> the lambda EcoR1 mwt markers.
> Maybe it will work.

Good idea, the same thing works for lambda x HindIII. The 4.6 kb fragment
contains one of the cos ends and so anneals with the other one.

-- 
Dr. Colin Rasmussen
Department of Anatomy & Cell Biology
University of Alberta
Edmonton, AB, Canada T6G 2H7.



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