ApoE typing by PCR
geneh at USA.NET
Mon Jan 9 19:37:22 EST 1995
We routinely use the primers and methods of Hixson and Vernier (J lipid
Res, 31: 545-548, 1990) for apoE genotyping (E2, E3, and E4) without too
many problems. Its based on restriction isotyping using amplification and
Hha I digestion. Email me if you need more info.
>I am trying to determine the ApoE status of several DNA samples using PCR
>to amplify a region of the ApoE gene. Using the primers and conditions of
>Wenham, Price and Blundell (Lancet 337:1158, 1991) we have been unable to
>amplify a single band of the correct size.
> Can anyone offer any help on conditions or primers which they have had
>success in using?
** Gene Holowachuk | Voice: 607/547-3937 **
** Research Institute | Fax : 607/547-3061 (attn Gene H) **
** Mary Imogene Bassett Hospital | email: geneh at research.mibh.org **
** One Atwell Road | : geneh at usa.net **
** Cooperstown, NY 13326 | : geneh at cscns.com **
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