Low Melt bands are contaminated/The Answer!

Peter pemanuel at umabnet.ab.umd.edu
Mon Jan 9 12:04:02 EST 1995


In article <pemanuel-0501950943340001 at beaker.ab.umd.edu>,
pemanuel at umabnet.ab.umd.edu (Peter) wrote:

> I recently ran a 1% low melt gel isolation of a 700 bp PCR fragment. When
> I cut out the band and isolated it with GENECLEAN I found that a
> contaminating band of 1400 bp had gotten in there somehow.
>    Thanks for your suggestions on this one.
>     Peter

I got a lot of responses on this one and I am posting to thank all of
you.  The answer is Yes it was a dimer-Heating to 65 degrees before
loading completely removed the dimer.  Chalk up one for the information
superhighway!
  Thanks again,
  Peter



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