5 min ligation
drm21 at mole.bio.cam.ac.uk
Mon Jan 9 14:36:29 EST 1995
> >Dave Boyd wrote:
> >Anybody try Boehringer Mannheim's 5 minute ligation kit yet?
> >Whats the secret ingredient? Does it really work?
> >Dave Boyd
> >University of Manitoba
What's the big deal with this? I've always done sticky-end ligations for
at room temperature (using NEB ligase) and they work really well. Lots
Even for 3 or 4-way ligations. I usually use 1hr for blunt end ligations
I'm not sure I'd make a library this way though - after all, they are so
that you might as well take the extra time just in case you get more
one day I should do a side-by-side 15min vs overnight, but since its works
for the stuff I do I've never bothered.
Try it, you'll like it!
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