5 min ligation
paolo de marco
lsraj at csv.warwick.ac.uk
Mon Jan 9 14:13:13 EST 1995
In article <84652160105991.440720 at usa>,
polli~j at glaxo.com ("Joseph Polli") writes:
>[This message is converted from WPS-PLUS to ASCII]
>Dave Boyd wrote:
>Anybody try Boehringer Mannheim's 5 minute ligation kit yet?
>Whats the secret ingredient? Does it really work?
>University of Manitoba
> I have used the Boehringer Mannheim's 5 min. ligation
>for several months now and it has worked very well. I
>think you should give it a try.
>Dept. Molecular Biology
>Glaxo Research Institute
>RTP, NC 27709
what kind of efficiencies do you get ? do you have to use bigger amounts of
DNA ?? would it be suited for any kind of ligation (like when making a
library for example ??)
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