5 min ligation

paolo de marco lsraj at csv.warwick.ac.uk
Mon Jan 9 14:13:13 EST 1995


In article <84652160105991.440720 at usa>,
	polli~j at glaxo.com ("Joseph Polli") writes:
>[This message is converted from WPS-PLUS to ASCII]
>
>Dave Boyd wrote:
>Anybody try Boehringer Mannheim's 5 minute ligation kit yet? 
>Whats the secret ingredient? Does it really work?
>
>Dave Boyd
>University of Manitoba                  
>
>
>Dave,
>
>    I have used the Boehringer Mannheim's 5 min. ligation
>for several months now and it has worked very well.  I 
>think you should give it a try.
>
>Joe Polli
>Dept. Molecular Biology
>Glaxo Research Institute
>RTP, NC  27709


what kind of efficiencies do you get ?   do you have to use bigger amounts of
DNA ??   would it be suited for any kind of ligation (like when making a 
library for example ??)

thanks                  paolo



More information about the Methods mailing list