TCA-precipitation?

K. Legate g9013118 at mcmail.cis.mcmaster.ca
Tue Jan 10 14:51:27 EST 1995


In article <3euc18$3ib at sunserver.lrz-muenchen.de>,
Peter Alliger <u7q11aa at sunmail.lrz-muenchen.de> wrote:
>I am looking for good method to precipitate a highly purified protein
>before 
>analysing it on a SDS-PAGE.
>  
I have had no problems TCA precipitating from high salt solutions. I 
simply resuspend the EtOH:ether washed pellet directly in SDS-PAGE 
loading buffer.

kyle





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