Lambda promoter plasmids

Duncan Clark Duncan at
Wed Jan 11 10:36:46 EST 1995

Hi Folks,

I have a query about the stability of lambda promoter expression 
plasmids. I have a plasmid with a lambda pl promoter, polylinker then
lambda terminator in that order. Normally I grow it in a cI ts E.coli 
or cI+ E.coli. One of my group just changed the polylinker and put it 
(without thinking) into a cI minus E.coli. It grows fine! Now I have
always been under the impression that lambda PL was lethal on plasmids
unless the cI repressor was present. Is there any truth to this. I am 
checking the map etc of the new construct just in case the promoter has 
been deleted. The plasmid is based on pBR322 with Amp not Tet.

Many thanks


My mind's made up. Don't confuse me with the facts!

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