MAXIMUM PCR DNA TEMPLATES?

JIM JIM
Fri Jan 13 08:36:39 EST 1995


HELP!
 
Yet more questions concerning PCR

Does any one know what the maximum amount of DNA templatethat you can
put in PCR reaction is?
We tried 10 µg but the results were not consistant (ie failed reactions)
5µg appeared to work quite well. 
Why does the increased amount of DNA template effect the reaction? The 
primers, bases taq etc are always well in excess.
Does the increased genomic DNA 'clog' it up?
Any ideas?

Thanks for your advice

Jim Harvey




More information about the Methods mailing list