MAXIMUM PCR DNA TEMPLATES?
Fri Jan 13 08:36:39 EST 1995
Yet more questions concerning PCR
Does any one know what the maximum amount of DNA templatethat you can
put in PCR reaction is?
We tried 10 µg but the results were not consistant (ie failed reactions)
5µg appeared to work quite well.
Why does the increased amount of DNA template effect the reaction? The
primers, bases taq etc are always well in excess.
Does the increased genomic DNA 'clog' it up?
Thanks for your advice
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