dna extraction from seeds?
koebner at BBSRC.AC.UK
Fri Jan 13 08:09:17 EST 1995
In article <3ekuvv$9m3 at biosun.harvard.edu>, ayala at biosun.harvard.edu (Francisco J. Ayala) says:
we have used a straight ctab followed by single chloroform extract on wheat
flour or bits of crushed endosperm.
yields about 1mu gr per 25mg seed tissue. Some starch coprecipitates with
the nucleic acid, but remains undissolved in the TE and doesn't interfere
with either pcr or restriction digestion.
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