phosphotyrosine antibody ?

William Schountz tonys at fox.ksu.ksu.edu
Fri Jan 13 23:18:38 EST 1995


In article <3f5v87$jra at sunserver.lrz-muenchen.de>, u7q11aa at sunmail.lrz-muenchen.de (Peter Alliger) writes:
> Can anyone recomend a phosphotyrosine-antibody for western-blotting?
> There are several on the market but which ones are really good and how
> to use them (dilution?, secondary antibody?, detection?).
> 
> Thanks for help 
> 
> Pit
> ===============================================================
> Peter Alliger, Ph.D.                   
> Institute for Immunology                 
> University of Munich             
>   
I use 4G10 from Upstate Biotechnology (New York).  It's excellent for blotting
as well as immunoprecipitation.

Tony



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