phosphotyrosine antibody ?
tonys at fox.ksu.ksu.edu
Fri Jan 13 23:18:38 EST 1995
In article <3f5v87$jra at sunserver.lrz-muenchen.de>, u7q11aa at sunmail.lrz-muenchen.de (Peter Alliger) writes:
> Can anyone recomend a phosphotyrosine-antibody for western-blotting?
> There are several on the market but which ones are really good and how
> to use them (dilution?, secondary antibody?, detection?).
> Thanks for help
> Peter Alliger, Ph.D.
> Institute for Immunology
> University of Munich
I use 4G10 from Upstate Biotechnology (New York). It's excellent for blotting
as well as immunoprecipitation.
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