PCR problem:inverted repeats
5gf4srivasta at vms.csd.mu.edu
Fri Jan 13 13:39:18 EST 1995
Im trying to PCR a 3-4 Kb DNA fragment which contains 1.6 Kb of
inverted repeat sequence. I get a shorter product which would correspond
to a secondary DNA structure loop (DNA hybridizing to itself) hence the taq
is unable to read through the entire template. I have tried hot starts to
eliminate this but to no avail. Does any one out there have any suggestions?
Any helpful hint would be appreciated!
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