Sequencing plates problem
bckraev at aeolus.ethz.ch
Sun Jan 15 08:48:26 EST 1995
There is the "last chance" cleaning aid, called "chromsulfuric acid"
( Merck 2499, about $20 for 1 liter ), which in earlier time was used by all
chemists to clean glassware. It is a nasty stuff, makes holes in your coat and
damages most plastic surfaces (except teflon), however, the glass is cleaned
really excellently and all traces of silanization are removed. Having your glass
cleaned in this way, you should mark the plates so that it is one plate and
one particular surface of this plate that will be silanized in the future at
regular intervals, as other netters have suggested. Now, if you have ( really
HAVE TO) to dry the gel on the plate, there are two ways of doing it
reliably ( and a couple of less reliable ways also exist ):
1. Treat one of the plates with gamma-metacrylpropyloxysilane ( details on
request) and your gel will be covalently bound to this plate. There is no
problem at all to fix this gel and dry it with a hair dryer. However, recycling
of this plate needs a little effort, and that is why this method is not very
popular (though the band resolution is truly excellent).
2. After fixing, cover the gel with a large cellophane membrane (e.g.BioRad) and
secure it with a homemade frame and clamps. It will dry overnight at r.t. The
cellophane is rather expensive and cannot be re-used ( but the resolution is
I use a semi-fixing protocol which I posted earlier under "Fixing gels-problems"
but it doesnt allow drying on the glass plate.
Good luck, Sasha
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