Pfu

Domingos henrique D_henrique at icrf.icnet.uk
Mon Jan 16 04:51:18 EST 1995


Dear netters
Can I have the experienced oppinion of someone that uses routinely Pfu
polymerase for amplifyng products for cloning. Is it worth to prevent
mistakes in the PCR? I want to use the PCR product for cloning and then
expression in E.coli and am afraid of mistakes that can result in
truncated proteins.
Thanks
Domingos



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