phosphotyrosine antibody ?

[Benoit_Hebert at IAF.UQUEBEC.CA]

Peter Alliger (u7q11aa at sunmail.lrz-muenchen.de) wrote:
> Can anyone recomend a phosphotyrosine-antibody for western-blotting?
> There are several on the market but which ones are really good and how
> to use them (dilution?, secondary antibody?, detection?).


I used PY20 and got decent results. This "sample" wasn't commercial
but I believe ICN is now distributing the mAB at a very high cost...

Benoit

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Benoit_Hebert at iaf.uquebec.ca

Virology Research Center
Armand-Frappier Institute, Laval, Quebec
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