pBR322 replication region

Herbert Wyckoff hwyckoff at ASRR.ARSUSDA.GOV
Mon Jan 16 14:51:06 EST 1995


I would like to amplify out the origin of replication from pBR322 by PCR 
to clone into a vector I'm constructing.  Could anyone tell me the exact 
region that is necessary for replication?  Has anyone designed successful 
primers for PCR'ing out the pBR322 ori?  Any help would be appreciated.

Thanks,
Herb Wyckoff
HWYCKOFF at ASRR.ARS.USDS.GOV





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