B-gal transcription

Jillian Johnston johnstoj at acs.ucalgary.ca
Mon Jan 16 18:32:12 EST 1995

I am trying to make capped B-gal mRNA (3kb) for translation
in Xenopus embryos using an SP6 polymerase.  I can't get full
length transcripts.  I have tried upping the amount of rNTPs but
this didn't help.  Before I waste anymore reagents (esp. cap
analog) I'd like some advice on possible areas that I should try.
I have no trouble making transcripts up to 2kb.
Thanks in advance for your help.
Jill Johnston

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