Sequenase

Dan Diaz bl275 at cleveland.Freenet.Edu
Mon Jan 16 20:54:53 EST 1995


i suggest going over to the glycerol tolerant (tris-taurine) system for all
of your polyacrylamide analyses of DNA molecules.  we dilute sequenase as
suggested in the glycerol dilution buffer along with pyrophosphatase.  its
stable forever and allows you to use every bit of enzyme without waste.

i use the tris-taurine buffer when running thin polyacrylamide gels for the
analysis of oligos and polynucleotides.  its a fine buffer - i cant recall
when i last used TBE.

diz



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