PEG solutions?

[Andre Hamel]

I recommend filter sterilization (0.2 um filter unit) instead of 
autoclaving, and storing in sterile glass bottles.

Regarding 50% w/v solutions ... takes a few hours to dissolve. Thus, 
start early in morning ... if stirring overnight, tightly cover opening of 
vessel (erlenmyer flask is preferred)  with clingy plastic (Saran-type) wrap.

cheers,




                            ********************
  Andre Hamel                      email: hamel at cc.umanitoba.ca
  Manitoba Government                         tel: 204/945-7630
  Department of Agriculture                   FAX: 204/945-8062
  Veterinary Services Branch          
  Winnipeg, Manitoba, CANADA 
			  ********************


In <3fef40$93o at crcnis3.unl.edu> vwarwar at unlinfo.unl.edu (vitor warwar) writes:

>     Is it fine to autoclave PEG solutions? How is the best
>way to dissolve PEG (50% w/v).

>     Vitor Warwar / 406 Plant Sciences Hall / University of Nebraska
>     Lincoln NE 68593-0722 / USA / vwarwar at unlinfo.unl.edu
>_______________________________________________________________________