baculo recombinationseq. question

[clontech!paulki at NETCOM.COM]

Fred writes:
> I have this wonderful Xho1 site that can be changed into a
> perfect Pst1 site by a single GC swap in the middle of the site. 
> Now, the problem is that the Xho1 is positioned in the *middle* of 
> (one of the) the Ac recombinationsites.

There are several vectors that do not have any viral sequences upstream 
from the XhoI site you refer to. This still leaves approx. 2 kb of 
viral sequences which is sufficient for recombinantion. Our pBacPAK8 
& 9 vectors have even less viral sequences, approx. 1.3 kb, and still 
work very well. Changing XhoI to PstI should not be a problem.
-= Paul =- 

[These are my opinions and not necessarily CLONTECH's]
.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~.
! Paul Kitts                                               !
! CLONTECH Laboratories, Inc.                              !
! 4030 Fabian Way               Email: paulki at clontech.com !
! Palo Alto                     Phone: 415 424 8222 x415   !
! CA 94303-4607, U.S.A.         Fax:   415 424 1064        !
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~