f_jacobs at biomira.com
Tue Jan 17 13:42:21 EST 1995
>From: UUCP%"bl275 at cleveland.Freenet.Edu" 17-JAN-1995 01:48
>To: methods-and-reagents at net.bio.net
>Subj: Re: Sequenase
>i suggest going over to the glycerol tolerant (tris-taurine) system for all
>of your polyacrylamide analyses of DNA molecules. we dilute sequenase as
>suggested in the glycerol dilution buffer along with pyrophosphatase. its
>stable forever and allows you to use every bit of enzyme without waste.
>i use the tris-taurine buffer when running thin polyacrylamide gels for the
>analysis of oligos and polynucleotides. its a fine buffer - i cant recall
>when i last used TBE.
I can find taurine in the Sigma catalogue but not in the Red book or the M&R FAQ.
Could someone please post a recipe or reference for its use in acrylamide gels?
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