Viability of cells in PBS? How long?
leach at acs.bu.edu
Tue Jan 17 11:22:55 EST 1995
In article <3fgd1k$7tv at mserv1.dl.ac.uk>, psansom at hgmp.mrc.ac.uk (Mr. P.A.
> Anyone any ideas how long cell membranes retain their integrity in PBS?
> I'm incubating substrate with cells for 1-5 hours and want to know if the
> cells are releasing internal contents through permeablised membranes.
> I dont want to use medium because it contains things that interfere with
> the HPLC detection method.
> If any thoughts or suggestions for simple basal medium I'd be grateful.
> Thanks in advance
try an electrophysiology bath solution....cells sit in this for hours...
10mM HEPES (pH 7.2 with NaOH)
this is the external solution they use....i used it once to incubate cells
at room temperature (in presence of luciferin) for an hour or so...they
didnt appear to choke on it ;)
..... Martin Leach Email:leach at mbcrr.harvard.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants on your
USA head.....WIBBLE" -BLACKADDER
p.s. try BioMOO (virtual biology on the internet - telnet
My home page: http://188.8.131.52/Leach.html
More information about the Methods